Contents & Storage

Contents & Storage

Illumina-Supplied Consumables

Item

Quantity

Storage

STM (Make sure that all STM tubes indicate the same stain temperature on the label)

2 tubes

-15° to -25°C

XC4

310 ml

-15° to -25°C

XC2

2 tubes

-15° to -25°C

ATM

2 tubes

-15° to -25°C

RA1

If you are performing an FFPE protocol, use 30 µl per sample well; otherwise, use 46 µl per sample well

-15° to -25°C

FMS

If you are performing an FFPE protocol, use 1 tube per 96 samples; otherwise, use 2 tubes per 96 samples

-15° to -25°C

RPM

2 tubes
(per 96 samples)

-15° to -25°C

MSM

2 tubes
(per 96 samples)

-15° to -25°C

XC1

2 tubes

-15° to -25°C

RA1

10 ml

-15° to -25°C

TEM

2 tubes

-15° to -25°C

PM1

2 tubes (per 96 samples)

2° to 8°C

PB2

2 tubes

Room temperature

Te-Flow Flow-Through Chambers (with Black Frames, Spacers, Glass Back Plates, and Clamps)

1 (per BeadChip)

Room temperature

Multi-Sample BeadChip Alignment Fixture

1 (per 8 BeadChips)

Room temperature

Hyb Chamber inserts

8

Room temperature

PB1

550 ml (up to 8 BeadChips)

Room temperature

Wash Dish

2 (up to 8 BeadChips)

Room temperature

Wash Rack

1 (up to 8 BeadChips)

Room temperature

Alconox Powder Detergent

as needed

Room temperature

EtOH

as needed

Room temperature

MA1

2 tubes
(per 96 samples)

Room temperature

XC3

75 ml

Room temperature

Hyb Chambers

2

Room temperature

Hyb Chamber gaskets

2

Room temperature

PB1

310 ml

Room temperature

BeadChips

8

Room temperature

User-Supplied Consumables

Item

Quantity

Storage

95% formamide/1 mM EDTA

15 ml

-15° to -25°C

BCD plate with Bisulfite-converted DNA samples

1 plate

-15° to -25°C

Lambda DNA

See Instructions

2° to 8°C

0.1N NaOH

15 ml
(per 96 samples)

2° to 8°C

PicoGreen dsDNA quantitation reagent

See Instructions

2° to 8°C

Zymo EZ DNA Methylation kit (includes bisulfite-conversion reagent, dilution buffer, desulphonation buffer, elution buffer)

1 kit per 50 samples

1 kit per 200 samples

or

1 kit per 2 plates

Room temperature

Fluotrac 200 96-well flat-bottom plate

1 per Std DNA plate

1 per Sample DNA plate

Room temperature

Genomic DNA (for HD Methylation)

³ 500 ng for each bisulfite conversion reaction for manual process

 

³1000 ng for each bisulfite conversion reaction for the Automated process

Room temperature

EtOH

500 ml

Room temperature

96-well 0.2 ml skirted microplate

1–3 plates

Room temperature

1X TE

See Instructions

Room temperature

96-well 0.8 ml microtiter plate (MIDI)

1 plate

Room temperature

100% 2-propanol

40 ml (per 96 samples)

Room temperature

96-well 0.65 ml microtiter plate

1 per 96 samples

Room temperature