HiSeq 3000 FAQs

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  • Reagents and Flow Cells


  • A patterned flow cell has billions of ordered nanowells that are manufactured into the flow cell glass. The ordered wells allow for the generation of sequencing clusters in an ordered arrangement. Clusters are aligned more closely together, increasing the number of output reads and amount of sequence data generated.

    The HiSeq 3000/4000 patterned flow cell has eight lanes and the same general dimensions as a HiSeq high-output flow cell.

    Reagents and flow cells provided in the HiSeq 3000/4000 SBS Kit and the HiSeq 3000/4000 Cluster Kits are designed and optimized for the HiSeq 4000 and HiSeq 3000 systems. Reagent kits designed for other HiSeq systems are not compatible with the HiSeq 4000 and HiSeq 3000 systems.