Requirements & Compatibility

Requirements & Compatibility

When clustering Nextera Rapid Capture Enrichment libraries on the cBot and sequencing on the HiSeq 1000/2000, GAIIx, or in High Output mode on the HiSeq 1500/2500, new primers are required whether performing a non-indexed, single-indexed, or dual-indexed run. Use the TruSeq Dual Index Sequencing Primer Box for Paired End runs (catalog # PE-121-1003), which is good for a single run and contains the required primers for sequencing (HP10, HP11, HP12).

Sequencing libraries prepared with the Nextera Rapid Capture Enrichment kits on the MiSeq, NextSeq 500, or HiSeq 1500/2500 rapid run reagent kits does not require any additional sequencing primers. These primers are included in the MiSeq Reagent Kit, NextSeq 500 Kit, and HiSeq 1500/2500 rapid run reagent kits.

Quantifying a Nextera Rapid Capture Enrichment post-enriched library using a fluorometric method results in the following cluster densities. Results can vary based on your method of quantification.

Instrument

Mode/Version

Required Concentration (pM)

Expected Cluster Density (mm2)

MiSeq

 

v2*

8–12

800,000–900,000

v3

7–14

1,200,000–1,400,000

NextSeq

High-output Flowcell

3

200,000–240,000

Mid-output Flowcell

3

200,000–240,000

HiSeq

High Output

8-12

750,000–950,000

Rapid Run

8-12

750,000–950,000

 

* Used for Nextera Rapid Capture Custom Enrichment only

 

For more information, see the Illumina Version Compatibility Reference.