PhiX Control v3

Catalog IDs: FC-110-3001

Latest Updates

MiSeq System Denature and Dilute Libraries Guide
Indexed Sequencing Overview Guide

What are PhiX Control Libraries?

Control libraries generated from the PhiX virus serve as an effective control in sequencing runs. Characteristics of the PhiX genome provide several benefits.

  • Small—PhiX is a small genome, which enables quick alignment and estimation of error rates.
  • Diverse—The PhiX genome contains approximately 45% GC and 55% AT.
  • Well-Defined—PhiX has a well-defined genome sequence. Illumina cluster generation algorithms are optimized around a balanced representation of A, T, G, and C nucleotides.

Illumina PhiX Control v3 is a balanced and diverse library that can help mitigate sequencing challenges in unbalanced and low-diversity libraries. Illumina PhiX Control v3 is shipped as a ready-to-use 10 nM library.

View PhiX v3 data in BaseSpace from a MiSeq 2 x 250 cycle sequencing run: SAV data and Resequencing results.