Use the TruSeq Genotype Ne kit for targeted genotyping by sequencing for parentage, purity testing, and breeding decisions for any plant or animal species. The kit produces high-quality results for up to 5000 SNPs and medium to high sample volume (1000–100,000).
Illumina recommends using 50 ng high-quality genomic DNA (gDNA). Use a fluorescence-based method, such as Qubit dsDNA Assay Kit or PicoGreen to quantify DNA. For more information on quantifying and diluting input DNA, see the TruSeq Genotype Ne Reference Guide (document # 1000000033138).
The TruSeq Genotype Ne Kit supports up to 5000 amplicons per reaction. The number of amplicons in the multiplex reaction varies depending on the experiment design, and can range from 116–5000.
The protocol requires two different magnetic stands: one for pre-PCR procedures and one for post-PCR procedures.
The recommended loading concentration depends on the sequencing system. For recommendations and instructions, see the denature and dilute libraries guide or system guide for your instrument.
Use the TruSeq Amplicon BaseSpace App or the Local Run Manager Amplicon DS analysis module to analyze TruSeq Gentoype Ne libraries.