Contents & Storage

Contents & Storage

HiSeq SBS reagents are shipped in 2 boxes on dry ice. When you receive your kit, promptly store the kit components at the indicated temperature to ensure proper performance.

Reagents are labeled with a 3-letter acronym plus a numeric designator that indicates the kit size. For example, if you are using CMR-50, the CMR was provided in a 50-cycle kit.

 

200-Cycle SBS Kit Box 1

Quantity

Reagent

Description

Storage Temperature

1

PW1

Wash Buffer

-25°C to 30°C

1

ICB

Incorporation Mix Buffer

2°C to 8°C

1

SB1

High Salt Buffer

2°C to 8°C

2

SB2

Incorporation Wash Buffer

2°C to 8°C

1

SB3

Cleavage Buffer

2°C to 8°C

 

200-Cycle SBS Kit Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

1

CMR

Cleavage Mix Reagent

1

SRE

Enhanced Scan Mix Reagent

1

EDP

Enhanced DNA Polymerase

4

LFN36

Long Read Nucleotide Mix

 

50-Cycle SBS Kit Box 1

Quantity

Reagent

Description

Storage Temperature

1

PW1

Wash Buffer

-25°C to 30°C

1

ICB

Incorporation Mix Buffer

2°C to 8°C

1

SB1

High Salt Buffer

2°C to 8°C

2

SB2

Incorporation Buffer

2°C to 8°C

1

SB3

Cleavage Buffer

2°C to 8°C

 

50-Cycle SBS Kit Box 2, Store at -25°C to -15°C

Quantity

Reagent

Description

1

CMR

Cleavage Mix Reagent

1

SRE

Enhanced Scanning Mix Reagent

1

EDP

Enhanced DNA Polymerase

1

LFN36

Long Read Nucleotide Mix