Introducing Nextera Unique Dual Indexes

07/23/19


Illumina recently launched IDT® for Illumina® Nextera™ DNA Unique Dual Indexes (Nextera UDI) to expand the selection of indexes for multiplexing, and to provide new solutions to mitigate index hopping. Nextera UDIs are compatible with Nextera library preparation chemistry to generate dual indexed libraries (10 bp for each index).

Below is a summary of the compatibility of the current index offerings with Nextera library preparation kits.

Table 1. Current Nextera index offerings and Nextera library preparation kits.

Index Name Catalog Numbers Index Length Library
Compatibility
with BBN*
Compatible Library Prep Kits
Nextera XT Nextera
DNA Flex
Nextera Flex for
Enrichment
Nextera UD
Indexes (UDI)
20027213
20027214
20027215
20027216
10 bp
Nextera DNA CD
Indexes
20018707
20018708
8 bp
Nextera XT
Indexes
FC-131-1001
FC-131-2001
FC-131-2002
FC-131-2003
FC-131-2004
8 bp

*BBN: Bead Based Normalization, reagents included in Nextera XT library preparation kits.

Note:

  • Nextera UDIs have not been validated with legacy Nextera Rapid Capture products.
  • Because Nextera UDI are not compatible with bead-based normalization (BBN), use manual normalization.

Index sequences are found in the Illumina Adapter Sequences Document.