AmpliSeq for Illumina BRCA Panel Frequently Asked Questions

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  • Analysis

  • The DNA Amplicon analysis workflow can be used to perform alignment and variant calling.  Local Run Manager and BaseSpace Sequence Hub have apps available for analysis. The DNA Amplicon Analysis App is available on BaseSpace Sequence Hub. Further analysis can be performed on any variant calls using BaseSpace Variant Interpreter.  Local Run Manager has a similar DNA Amplicon Analysis Module which utilizes the same workflow and algorithm as the BaseSpace Sequence Hub Apps. 

    Yes, there are example data sets in BaseSpace Public Data.

    On-target bases shows the percentage of total sequenced bases that map to target regions in the reference genome. This metric reflects the percentage of bases from amplicons that a) were designed, synthesized, and pooled and b) generated sequence data mapping to the target regions.