AmpliSeq for Illumina Immune Response Panel FAQs

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  • Analysis

  • The RNA Amplicon analysis workflow can be used for differential expression analysis.  The RNA Amplicon Analysis App is available on BaseSpace Sequence Hub. Local Run Manager has a similar RNA Amplicon Analysis Module which utilizes the same workflow and algorithm as the BaseSpace Sequence Hub Apps.

    Yes, there are example data sets in BaseSpace Public Data.

    On-target bases shows the percentage of total sequenced bases that map to target regions in the reference genome. This metric reflects the percentage of bases from amplicons that a) were designed, synthesized, and pooled and b) generated sequence data mapping to the target regions.