TruSeq Nano DNA Library Prep Kit for NeoPrep FAQs

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  • On NeoPrep, TruSeq Nano DNA library prep starts with genomic DNA, which is sheared using a Covaris focused-ultrasonicator and low-volume Covaris microTUBE-15 tubes. Sheared DNA is then concentrated and bound with magnetic beads to load onto the NeoPrep library card. Bead cleanup before loading is not necessary.

    • For a 350 bp insert size, use 25 ng input DNA. Do not exceed 50 ng gDNA.
    • For a 550 bp insert size, use 75 ng input DNA. Do not exceed 150 ng gDNA.