TruSight Oncology 500 ctDNA FAQs

  • Input Sample Quantification

  • We recommend quantifying cfDNA before starting the assay using a capillary electrophoresis-based method, such as the Fragment Analyzer (Agilent 5300 system with High Sensitivity Large Fragment Analysis Kit) or TapeStation (Agilent 4200 TapeStation System with Agilent Cell-free DNA Screen Tape Assay).

    The Bioanalyzer is not recommended due to the peak quantification method used (based on both lower and upper marker). Quantification will be based on size which only includes the cfDNA fraction (75-250 bp on Fragment Analyzer and 125-300 bp on TapeStation) to ensure optimal input into the assay.