MiSeq Training

  • Introductory Topics


  • Sequencing: Fundamentals

    After completing this course you will be able to describe DNA, RNA and the central dogma; discuss traditional sequencing methodologies; and compare traditional sequencing to sequencing by synthesis.

    Languages: French, Italian, German, SpanishChinese (Simplified)

    15 min

  • How Do I Prepare for My Illumina Instrument Delivery?

    In this video, an Illumina Field Applications Scientist discusses what to expect during the pre-installation, shipping, and delivery stages. We’ll highlight important steps in the process and introduce you to available resources. Note that, depending on your region, the steps shown in this video may not apply to the iSeq 100 system.

    7 min

  • Library Prep


  • Illumina Experiment Manager

    This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.

    25 min

  • AmpliSeq for Illumina: Library Prep Protocol

    This course identifies the items needed for the AmpliSeq for Illumina library prep protocol, introduces pool planning resources for a multiplexed amplicon run, demonstrates the steps in the library prep protocol, and lists best practices for the protocol.

    30 min

  • AmpliSeq for Illumina: Overview

    This course describes the AmpliSeq for Illumina assay technology, lists the three types of panels, introduces the steps in the amplicon sequencing workflow, and lists the analysis options for amplicon sequencing data.

    15 min

  • Nextera DNA Flex Library Preparation

    By the end of this course, you will be able to identify the Nextera DNA Flex kit, describe how the protocol works, identify what is needed to complete the protocol, and define the various steps in the library preparation workflow.

    20 min

  • Instruments & Software


  • Discover the Benefits of Illumina Proactive

    Learn about the benefits of instrument performance monitoring through Illumina Proactive, a service in which instrument performance data are sent to Illumina to enable proactive maintenance.

    10 min

  • IT Requirements for Implementing Illumina Proactive

    This video shares IT requirements for implementing Illumina Proactive, an instrument performance monitoring service provided by Illumina that can increase instrument uptime, improve operational efficiency, and reduce the risk of lost resources.

    4 min

  • MiSeq: How to Start a Run

    After completing this course you will be able to identify the MiSeq run setup options, describe how to prepare the MiSeq reagent cartridge and load libraries, and list the steps to start a sequencing run with MiSeq Control Software (MCS).

    20 min

  • MiSeq: Instrument Washes

    This course provides an overview of the three MiSeq instrument washes: the post-run wash, the maintenance wash, and the standby wash.

    Languages: Spanish, Chinese (Simplified), Japanese

    10 min

  • MiSeq: Preparing for Your Install

    After completing this course you will be able to describe the lab environment required to use MiSeq, list key components of MiSeq, identify the network considerations for MiSeq, and list the user-supplied consumables required for running and maintaining MiSeq.

    20 min

  • MiSeq: Does My Run Look Good?

    After completing this course you will be able to list two options for monitoring your MiSeq run, describe key metrics in MiSeq Control Software (MCS), and describe key metrics in Sequencing Analysis Viewer (SAV).

    10 min

  • Local Run Manager 2.0 (Research Use Only): Introduction - (Asia Pacific - Chinese)

    Upcoming Webinar | Local Run Manager 2.0介绍

    Local Run Manager是Illumina专门针对所有小型桌面测序仪而推出的一款集合解决方案的windows软件,该软件可记录一个run的样本信息,设定run的运行参数,监控run的状态,分析测序数据以及对结果进行可视化。本次网络研讨会中,Illumina 技术支持会重点给您讲解RUO模式下以及off-instrument(可安装在个人电脑)版本的Local Run Manager 2.0软件,主要包括如何进行用户管理,创建一个run以及如何进行数据的分析。本次研讨会不涉及Dx模式下的Local Run Manager软件。讲座内容适用于所有对Illumina测序产品感兴趣的客户尤其是初次接触测序的用户。

    May 23, 2019 CST

  • Data Analysis


  • How Can I Tell If I Sequenced Through the Insert? Part 1

    An Illumina Field Applications Scientist describes what it means to sequence through the insert of your prepared library. We look at Bioanalyzer traces to assess the range of insert sizes in a library.

    7 min

  • How Can I Tell If I Sequenced Through the Insert? Part 2

    This video shows you how to use Sequencing Analysis Viewer (SAV) plots to determine if your sequencing insert is too short. We also explore how to use the adaptertrimming.txt file on the MiSeq to diagnose short inserts.

    5 min

  • Is My HiSeq or MiSeq Run Overclustered?

    This video provides an overview of overclustering and how it can impact your sequencing data. An Illumina Field Applications Scientist shows you how to use Sequencing Analysis Viewer (SAV) to look for common symptoms of an overclustered HiSeq or MiSeq flow cell.

    7 min

  • MiSeq: Data Analysis Overview

    After completing this course you will be able to list the MiSeq data analysis tools, briefly describe the function of each tool, and list some considerations when choosing your MiSeq data analysis tool.

    Languages: SpanishJapanese

    10 min

  • MiSeq: Imaging and Base Calling

    After completing this course you will have an understanding of how the MiSeq processes images to extract intensities and processes intensities to extract base calls.  These processes are completed automatically by the MiSeq.

    Languages: Spanish, Chinese (Simplified), Japanese

    35 min

  • MiSeq: MiSeq Reporter

    After completing this course you will have an understanding of the demultiplexing and alignment processes and program interfaces that are common to all workflows in MiSeq Reporter. You will also be able to briefly describe the utility of the supported MiSeq Reporter workflows.

    Languages: Spanish, Chinese (Simplified), Japanese

    25 min