Training

Introductory Courses

Online Course Title Description Length
MiSeq: Sequencing Chemistry

After completing this course you will be able to describe the Illumina sequencing workflow, discuss the clustering process, and define the sequencing process.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
MiSeq: Sequencing Fundamentals

After completing this course you will be able to describe DNA, RNA and the central dogma; discuss traditional sequencing methodologies; and compare traditional sequencing to Illumina's sequencing chemistry.

Languages: Spanish, Japanese

15 min
Sequencing: Illumina Technology

This course provides a general overview of the Illumina sequencing workflow from extraction of nucleic acids to the completion of a sequencing run.

Languages: Spanish, Chinese (Simplified)

30 min
Sequencing: Illumina's Dual Indexing Strategy

The goal of dual index sequencing is to increase the multiplex level of sequencing per lane, so that more samples can be sequenced on the same flow cell.

Languages: Spanish, Chinese (Simplified), Japanese

10 min

Sample Prep

Online Course Title Description Length
DesignStudio: TruSeq Targeted RNA Expression

After completing this course you will be able to create a project using DesignStudio Gene Expression.

Languages: Spanish, Chinese (Simplified), Japanese

10 min
Illumina Experiment Manager

This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.

25 min
Nextera DNA Sample Prep

After completing this course you will be able to describe the items necessary to run Nextera DNA sample preparation assay to generate MiSeq sequence-ready libraries, describe the steps in Nextera DNA sample preparation workflow, and identify some of the assay's most important considerations.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
Nextera Rapid Capture

After completing this course, you will be able to identify the Nextera Rapid Capture Enrichment kits, choose your Nextera Rapid Capture Enrichment Product, identify how the protocol works, describe what is needed to successfully complete the protocol, define the various steps in the Sample Preparation workflow, and analyze Nextera Capture Enrichment data.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
Nextera Sample Prep: Best Practices

After completing this course you will be able to determine appropriate starting material, perform critical techniques used in the sample prep process, and accurately validate the final libraries. Note that the best practices presented in this course do not apply to the Nextera™ DNA Flex Library Prep Kit.

 

30 min
TruSeq Amplicon - Cancer Panel

By the end of this training, you will be able to describe the items necessary to run the TruSeq Amplicon Cancer Panel assay, describe the steps in the sequencing workflow, identify some of the best practices, and set up a sample sheet for sequencing.

 

20 min
TruSeq Targeted RNA Expression Kit

After completing this course you will be able to describe the TruSeq Targeted RNA Expression Kit, the steps to order a custom oligo pool, the steps in the TruSeq Targeted RNA Expression protocol, best practices, and how to analyze and normalize data.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
TruSeq: Best Practices

After completing this course you will understand best practices for all TruSeq Sample Preparation Kits including liquid handling, AMPure XP handling, avoiding cross-contamination, and temperature considerations. You will also learn about specific information about DNA and RNA best practices.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
TruSeq: Comparison of Available DNA Sample Preparation Kits

After completing this course you will know the similarities and differences between currently available TruSeq DNA Sample Prep Kits, the starting input amounts and final library characteristics for the various kits, how the various protocols differ, and the ordering information for the various TruSeq DNA kits.

 

20 min
TruSeq: Controls

By the end of this course you will be able to identify controls, describe the different SAV reporting types, and understand how to support these controls.

Languages: Spanish, Chinese (Simplified), Japanese

15 min
TruSeq: DNA PCR-Free Library Prep

Topics included in this course include the TruSeq DNA PCR-Free library prep protocol, what the protocol does, how the protocol works, what is needed to complete the protocol successfully, and the various steps in the workflow.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
TruSeq: DNA Sample Prep

After completing this course you will know the TruSeq DNA Sample Prep Protocol, what the protocol does, how the protocol works, what is needed to successfully complete the protocol, and the various steps in the Sample Prep Workflow.

 

10 min
TruSeq: Nano DNA Sample Prep

After completing this course, you will know: what the TruSeq Nano DNA Sample Prep Protocol is, what the protocol does, how the protocol works, what is needed to successfully complete the protocol, and the various steps in the sample prep workflow.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
TruSeq: Sample Purification Bead Size Selection and Best Practices

After completing this course you will understand what Sample Purification Beads are, how the Sample Purification Beads work, when Sample Purification Beads are used, what the Size Selection Workflow is, and the Sample Purification Beads best practices.

 

20 min
TruSeq: Small RNA Sample Prep

After completing this course you will know the TruSeq Small RNA Sample Prep Protocol, what the protocol does, how the protocol works, what is needed to successfully complete the protocol, and the various steps in the Sample Prep Workflow.

Languages: Spanish, Chinese (Simplified), Japanese

10 min
TruSeq: Stranded RNA Sample Prep

After completing this course, you will learn answers to questions including: What is the TruSeq Stranded mRNA Sample Prep Protocol? and What is the TruSeq Stranded Total RNA Sample Prep Protocol

Languages: Spanish, Chinese (Simplified), Japanese

20 min
DesignStudio: TruSeq Custom Amplicon

This course provides an introduction to the DesignStudio application by walking the user through ordering a experiment.

Languages: Japanese

20 min
TruSeq: Custom Amplicon Library Prep

After completing this course you will be able to describe the items necessary to run the TruSeq Custom Amplicon assay, list the steps in the workflow, and identify the best practices.

Languages: Spanish, Chinese (Simplified), Japanese

20 min

Instruments & Software

Online Course Title Description Length
MiSeq: Getting Started

After completing this course you will be able to describe the lab environment required to use MiSeq, list key components of MiSeq, and list the user-supplied consumables required for running and maintaining MiSeq.

Languages: Spanish, Chinese (Simplified), Japanese

20 min
MiSeq: How to Start a Run

After completing this course you will be able to list the library construction methods available for MiSeq, create an electronic sample sheet, prepare and load MiSeq reagents, and start a sequencing run using MiSeq Control Software (MCS).

Languages: Spanish, Chinese (Simplified), Japanese

20 min
MiSeq: Instrument Washes

This course provides an overview of the three MiSeq instrument washes: the post-run wash, the maintenance wash, and the standby wash.

Languages: Spanish, Chinese (Simplified), Japanese

10 min
MiSeq: Introduction to the MiSeq System

After completing this course, you will be able to describe the simplified sequencing workflow on the MiSeq, understand the features of MiSeq, and list the six MiSeq sequencing workflows.

Languages: Spanish, Chinese (Simplified), Japanese

20 min

Data Analysis

Online Course Title Description Length
MiSeq: Data Analysis Overview

After completing this course you will be able to list the MiSeq data analysis tools, briefly describe the function of each tool, and list some considerations when choosing your MiSeq data analysis tool.

Languages: SpanishJapanese

10 min
MiSeq: Does My Run Look Good?

After completing this course you will be able to list two options for monitoring your MiSeq run, describe key metrics in MiSeq Control Software (MCS), and describe key metrics in Sequencing Analysis Viewer (SAV).

10 min
MiSeq: Imaging and Base Calling

After completing this course you will have an understanding of how the MiSeq processes images to extract intensities and processes intensities to extract base calls.  These processes are completed automatically by the MiSeq.

Languages: Spanish, Chinese (Simplified), Japanese

35 min
MiSeq: MiSeq Reporter

After completing this course you will have an understanding of the demultiplexing and alignment processes and program interfaces that are common to all workflows in MiSeq Reporter. You will also be able to briefly describe the utility of the supported MiSeq Reporter workflows.

Languages: Spanish, Chinese (Simplified), Japanese

25 min
BaseSpace Sequence Hub: FAQs

This course helps you to locate answers to commonly asked questions about BaseSpace Sequence Hub.

25 min
BaseSpace Sequence Hub: Introduction

The course explores the BaseSpace Sequence Hub interface and its key features.

20 min