Kit Requirements & Contents

NextSeq 500/550 Kit Requirements

A single-use NextSeq 500/550 Kit is required to perform a sequencing run.

Requirements & Compatibility

Flow Cell Types

Use the appropriate reagent cartridge for a given flow cell type. Each reagent cartridge and flow cell is marked with either High or Mid for easy identification. The buffer cartridge is universal.

Kit Name

Flow Cell Type

Number of Tiles

NextSeq 500/550 High Output Kit

TG NextSeq 500 High Output Kit

NextSeq high output flow cell

864 tiles

NextSeq 500/550 Mid Output Kit

NextSeq mid output flow cell

288 tiles

Contents & Storage






Reagent Cartridge*

-25°C to -15°C

Contains clustering and sequencing reagents


Buffer Cartridge*

15°C to 30°C

Contains buffers and wash solution


Flow Cell**

2°C to 8°C

Single-use flow cell



-25°C to -15°C

Hybridization Buffer

* Reagents are sensitive to light. Store the reagent cartridge and buffer cartridge in a dark location away from light.

** NextSeq v2.5 flow cells are shipped at ambient temperature; once received, store the flow cell at 2° - 8°.

† HT1 is used to dilute libraries before sequencing. For more information, see NextSeq System Denature and Dilute Libraries Guide (part # 15048776).