IDT for Illumina DNA/RNA UD Indexes Compatible Products

Product Description

IDT for Illumina DNA/RNA UD Indexes are extension-based unique dual (UD) indexes with unrelated adapters for both index reads. The index adapter sequences are 10 bases long.

Sample Information

Before preparing libraries, create a sample sheet to record the index adapter combinations and other information about your samples. Sample sheet options and requirements depend on the instrument. For compatibility information, see the support pages for your instrument.

When using Local Run Manager or Illumina Experiment Manager (IEM) to prepare a sample sheet, consider the following requirements for IDT for Illumina DNA/RNA UD Indexes:

• Local Run Manager or Local Run Manager for MiniSeq—Add the kit to Local Run Manager by importing the appropriate Local Run Manager Library Prep Kit Definition File (*.tsv) or Local Run Manager Sample Sheet (*.csv) into Local Run Manager. For instructions, see the Local Run Manager Software Guide.
• Illumina Experiment Manager—Use IEM v1.16 or later. Earlier versions do not include the indexes. You can also download the appropriate IEM sample sheet template.

Library Prep

IDT for Illumina DNA/RNA UD Indexes are compatible with the following library prep kits.

• Illumina DNA Prep
• Illumina DNA Prep with Enrichment
• Nextera XT DNA (standard normalization only)
• Illumina DNA PCR-Free
• lIllumina RNA Prep with Enrichment
• Illumina Stranded mRNA Prep
• lllumina Stranded Total RNA Prep
• TruSight Oncology 500 High Throughput
• TruSight Oncology 500 ctDNA v2

Sequencing

Compatible Instruments

The following instruments can sequence libraries appended with IDT for Illumina DNA/RNA UD Indexes. For library prep kit and instrument compatibility, see the support pages for your kit or instrument.

• HiSeq 2500, HiSeq 3000, and HiSeq 4000 Systems
• MiniSeq System
• MiSeq System
• NextSeq 500 and NextSeq 550 Systems
• NovaSeq 6000 System

Read Lengths

Enter 10 cycles each for Index 1 and Index 2 to ensure that the software reads the complete 10-base index adapter sequence. For the recommended number of Read 1 and Read 2 cycles, see the Compatible Products page for your kit.

• Illumina DNA Prep Compatible Products
• Illumina DNA Prep with Enrichment Compatible Products
• Nextera XT DNA Compatible Products

The system guide for each instrument provides instructions for entering cycles for Read 1, Index 1, Index 2, and Read 2.