HiSeq 3000 Training

  • Introductory Topics

    DATE/LENGTH


  • Bcl2Fastq v2+: Introduction

    Recorded Webinar (April 2019) | Bcl2Fastq is a Linux-based software that converts base call files generated from an Illumina sequencing run to FASTQ files as well as demultiplex samples. This webinar focuses on Bcl2fastq v2+ and is targeted for new to intermediate users who are interested in the configurable options that this command line program allows. We will cover the following topics: requirements to run Bcl2Fastq, command line basics, commonly used options, frequently asked ‘How To….’ questions.


  • Sequencing: Illumina Technology

    This course provides a general overview of the Illumina sequencing workflow from extraction of nucleic acids to the completion of a sequencing run.

    Languages: French, Italian, German, SpanishChinese (Simplified), French,

    15 min


  • Sequencing: Introduction to Sequencing By Synthesis (SBS)

    Recorded Webinar (July 2019) | Illumina next-generation sequencing technology allows for massive parallel sequencing. Illumina Technical Support invites you to join us for a presentation and discussion on sample preparation, cluster generation and sequencing by synthesis chemistry. This webinar is targeted to those new to next-generation sequencing and will discuss the following topics: Illumina library construction, cluster generation methods by platform, sequencing by synthesis, and multiplexing and primary analysis.


  • Sequencing: Introduction to Sequencing By Synthesis (SBS) - Asia Pacific (Chinese)

    Upcoming Webinar | Illumina Technical Support 团队邀请您参加一场有关文库制备,簇生成和边合成边测序原理的研讨会。目标人群为二代测序的新用户,通过本次网络研讨会可以对测序原理有更深入的了解。讲座内容涵盖以下方面: Illumina文库结构组成,簇生成过程,不同平台上边合成边测序原理,Index测序策略,数据分析简介。

    Aug 14, 2019 CST


  • Library Preparation

    DATE/LENGTH


  • Illumina Experiment Manager

    This course provides an introduction to Illumina Experiment Manager, and shows you the steps to create sample plates and sample sheets.

    25 min


  • Data Analysis

    DATE/LENGTH


  • How Can I Tell If I Sequenced Through the Insert? Part 1

    An Illumina Field Applications Scientist describes what it means to sequence through the insert of your prepared library. We look at Bioanalyzer traces to assess the range of insert sizes in a library.

    7 min


  • How Can I Tell If I Sequenced Through the Insert? Part 2

    This video shows you how to use Sequencing Analysis Viewer (SAV) plots to determine if your sequencing insert is too short. We also explore how to use the adaptertrimming.txt file on the MiSeq to diagnose short inserts.

    5 min


  • Is My HiSeq or MiSeq Run Overclustered?

    This video provides an overview of overclustering and how it can impact your sequencing data. An Illumina Field Applications Scientist shows you how to use Sequencing Analysis Viewer (SAV) to look for common symptoms of an overclustered HiSeq or MiSeq flow cell.

    7 min