This guide provides lab specifications and requirements for preparing the lab for sequencing on the HiSeq 2500, HiSeq 1500, or HiSeq 2000 system.
The safety and compliance guide describes safety considerations and lists product compliance markings for the HiSeq 1500 and HiSeq 2500 systems.
This guide contains safety information and product compliance statements for the HiSeq.
This guide contains instructions for denaturing and diluting libraries after library preparation and before sequencing on a HiSeq or GAIIx system. This guide also includes instructions for preparing a PhiX control.
This guide provides an overview of instrument components and instructions for operating and maintaining the HiSeq 2000.
Instructions for performing Read 1 primer rehybridization on a TruSeq v3 (HiSeq) or TruSeq v2 (GAIIx) flow cell using the TruSeq v2 cBot Multi-Primer Re-Hybridization Kit.
This guide contains instructions for performing primer rehybridization on the HiSeq using the HiSeq Multi-Primer Rehyb Kit v4. Instructions for a TruSeq v3 Read 2 primer rehybridization are also included.
This guide contains instructions for using custom primers for a sequencing run on the HiSeq 2500, HiSeq 1500, HiSeq 2000, HiSeq 3000, or HiSeq 4000 system.
This guide contains instructions for sequencing dual-indexed libraries on the HiSeq.
This guide provides an overview of the indexed sequencing process for single-indexing and dual-indexing workflows on the iSeq, NovaSeq, MiniSeq, NextSeq, MiSeq, and HiSeq sequencing systems.