Contents & Storage

Each Nextera XT DNA Library Prep Kit (24 or 96 sample) has a corresponding Index Kit that contains 24 or 96 indexes (separately ordered). A Nextera XT Index kit is required to complete the protocol, regardless of the sample pooling level used for sequencing. 

With the introduction of the Nextera XT Index Kit v2, catalog no. FC-131-1002 has been obsolesced (Nextera XT Index Kit - 96 indexes, 384 samples).

Nextera XT DNA Sample Preparation Kit for 24 Samples (FC-131-1024)

Box 1

Reagent

Storage

Description

ATM

-25° to -15°C

Amplicon Tagment Mix, 96 RXN

TD

-25° to -15°C

Tagment DNA Buffer

NPM

-25° to -15°C

Nextera PCR Master Mix

RSB

-25° to -15°C

Resuspension Buffer

LNA1

-25° to -15°C

Library Normalization Additives 1

LNW1

2° to 8°C

Library Normalization Wash 1

HT1

-25° to -15°C

Hybridization Buffer

Box 2

Reagent

Storage

Description

NT

Room temperature

Neutralize Tagment Buffer

LNB1

2° to 8°C

Library Normalization Beads 1

LNS1

Room temperature

Library Normalization Storage Buffer 1

Bead-Based Normalization Compatible Index Kits

Nextera XT Index Kit for 24 Indexes, 96 Samples (FC-131-1001)

Reagent

Storage

Description

Index Primers

-25° to -15°C

S502 to S504, and S517

Index Primers

-25° to -15°C

N701 to N706

Nextera XT DNA Sample Preparation Kit for 96 Samples (FC-131-1096)

Box 1

Reagent

Storage

Description

ATM

-25° to -15°C

Amplicon Tagment Mix, 96 RXN

TD

-25° to -15°C

Tagment DNA Buffer

NPM

-25° to -15°C

Nextera PCR Master Mix

RSB

-25° to -15°C

Resuspension Buffer

LNA1

-25° to -15°C

Library Normalization Additives 1

LNW1

2° to 8°C

Library Normalization Wash 1

HT1

-25° to -15°C

Hybridization Buffer

Box 2

Reagent

Storage

Description

NT

Room temperature

Neutralize Tagment Buffer

LNB1

2° to 8°C

Library Normalization Beads 1

LNS1

Room temperature

Library Normalization Storage Buffer 1

Nextera XT Index Kit for 96 Indexes, 384 Samples (FC-131-1002)

Reagent

Storage

Description

Index Primers

-25° to -15°C

S502 to S508, and S517

Index Primers

-25° to -15°C

N701 to N712

Nextera XT Index Kit v2 Set A for 96 Indexes, 384 Samples (FC-131-2001)

Reagent

Storage

Description

Index Primers

-25° to -15°C

S502, S503, S505 to S508, S510, and S511

Index Primers

-25° to -15°C

N701 to N707, N710 to N712, N714, and N715

Nextera XT Index Kit v2 Set B for 96 Indexes, 384 Samples (FC-131-2002)

Reagent

Storage

Description

Index Primers

-25° to -15°C

S502, S503, S505 to S508, S510, and S511

Index Primers

-25° to -15°C

N716, N718 to N724, and N726 to N729

Nextera XT Index Kit v2 Set C for 96 Indexes, 384 Samples (FC-131-2003)

Reagent

Storage

Description

Index Primers

-25° to -15°C

S513, S515 to S518, and S520 to S522

Index Primers

-25° to -15°C

N701 to N707, N710 to N712, N714, and N715

Nextera XT Index Kit v2 Set D for 96 Indexes, 384 Samples (FC-131-2004)

Reagent

Storage

Description

Index Primers

-25° to -15°C

S513, S515 to S518, and S520 to S522

Index Primers

-25° to -15°C

N716, N718 to N724, and N726 to N729

Standard Normalization Compatible Index Kits (Manual Normalization Only)

IDT for Illumina Nextera DNA UD Indexes – Set A (96 Indexes, 96 Samples), Store at -25°C to -15°C

For more information, see IDT for Illumina Nextera DNA UD Indexes.