Contents & Storage

Each Nextera XT DNA Library Prep Kit (24 or 96 sample) has a corresponding Index Kit that contains 24 or 96 indexes (separately ordered). A Nextera XT Index kit is required to complete the protocol, regardless of the sample pooling level used for sequencing. 

Nextera XT DNA Sample Preparation Kit for 24 Samples (FC-131-1024)

Box 1

Reagent

Storage

Description

ATM

-25° to -15°C

Amplicon Tagment Mix, 96 RXN

TD

-25° to -15°C

Tagment DNA Buffer

NPM

-25° to -15°C

Nextera PCR Master Mix

RSB

-25° to -15°C

Resuspension Buffer

LNA1

-25° to -15°C

Library Normalization Additives 1

LNW1

2° to 8°C

Library Normalization Wash 1

HT1

-25° to -15°C

Hybridization Buffer

Box 2

Reagent

Storage

Description

NT

Room temperature

Neutralize Tagment Buffer

LNB1

2° to 8°C

Library Normalization Beads 1

LNS1

Room temperature

Library Normalization Storage Buffer 1

Bead-Based Normalization Compatible Index Kits

Nextera XT Index Kit for 24 Indexes, 96 Samples (FC-131-1001)

Reagent

Storage

Description

Index Primers

-25° to -15°C

S502 to S504, and S517

Index Primers

-25° to -15°C

N701 to N706

Nextera XT DNA Sample Preparation Kit for 96 Samples (FC-131-1096)

Box 1

Reagent

Storage

Description

ATM

-25° to -15°C

Amplicon Tagment Mix, 96 RXN

TD

-25° to -15°C

Tagment DNA Buffer

NPM

-25° to -15°C

Nextera PCR Master Mix

RSB

-25° to -15°C

Resuspension Buffer

LNA1

-25° to -15°C

Library Normalization Additives 1

LNW1

2° to 8°C

Library Normalization Wash 1

HT1

-25° to -15°C

Hybridization Buffer

Box 2

Reagent

Storage

Description

NT

Room temperature

Neutralize Tagment Buffer

LNB1

2° to 8°C

Library Normalization Beads 1

LNS1

Room temperature

Library Normalization Storage Buffer 1

Nextera XT Index Kit for 96 Indexes, 384 Samples (FC-131-1002)

Reagent

Storage

Description

Index Primers

-25° to -15°C

S502 to S508, and S517

Index Primers

-25° to -15°C

N701 to N712

Nextera XT Index Kit v2 Set A for 96 Indexes, 384 Samples (FC-131-2001)

Reagent

Storage

Description

Index Primers

-25° to -15°C

S502, S503, S505 to S508, S510, and S511

Index Primers

-25° to -15°C

N701 to N707, N710 to N712, N714, and N715

Nextera XT Index Kit v2 Set B for 96 Indexes, 384 Samples (FC-131-2002)

Reagent

Storage

Description

Index Primers

-25° to -15°C

S502, S503, S505 to S508, S510, and S511

Index Primers

-25° to -15°C

N716, N718 to N724, and N726 to N729

Nextera XT Index Kit v2 Set C for 96 Indexes, 384 Samples (FC-131-2003)

Reagent

Storage

Description

Index Primers

-25° to -15°C

S513, S515 to S518, and S520 to S522

Index Primers

-25° to -15°C

N701 to N707, N710 to N712, N714, and N715

Nextera XT Index Kit v2 Set D for 96 Indexes, 384 Samples (FC-131-2004)

Reagent

Storage

Description

Index Primers

-25° to -15°C

S513, S515 to S518, and S520 to S522

Index Primers

-25° to -15°C

N716, N718 to N724, and N726 to N729

Standard Normalization Compatible Index Kits (Manual Normalization Only)

IDT for Illumina Nextera DNA UD Indexes – Set A (96 Indexes, 96 Samples), Store at -25°C to -15°C

For more information, see IDT for Illumina Nextera DNA UD Indexes.