A comprehensive list of resources needed to set up, operate, maintain, and troubleshoot the HiSeq™ 3000 and HiSeq 4000 sequencing systems is on the HiSeq 3000 and HiSeq 4000 Support pages. Specific documentation links can be found below.

• HiSeq 3000/HiSeq 4000 Brochure
  Introductory brochure to the HiSeq 3000 and 4000 systems.
• HiSeq 4000 and HiSeq 3000 Systems Site Prep Guide
  Includes information for lab specifications and requirements to prepare a site for the HiSeq 3000 and HiSeq 4000 systems.
• HiSeq 3000 System Guide and HiSeq 4000 System Guide
  System overview and instructions for operating and maintaining the HiSeq 3000 and HiSeq 4000 instruments.
• HiSeq 3000/4000 System quality and performance specifications
  Run specifications can be found here, including expected data yield, quality, and run time for a given run length on HiSeq 3000 and HiSeq 4000 systems.
• HiSeq 3000/4000 Specification Sheet
  Introduces patterned flow cells, specifications for the instrument control computer, operating environment, laser specifications, instrument dimensions, and power requirements.
• Read 1 Primer Rehyb on a HiSeq 3000/4000 Flow Cell
  Instructions for performing Read 1 primer rehybridization on a HiSeq 3000/4000 flow cell using the HiSeq 3000/4000 cBot Multi-Primer Rehybridization Kit.
• Calculating Percent Passing Filter for Patterned and Nonpatterned Flow Cells
  Technical Note outlines differences between percent passing filter calculation for patterned and nonpatterned flow cells.
• Online Training Courses for HiSeq 3000 and Online Training Courses for HiSeq 4000
  Online trainings and recorded webinars include:
  • An overview of ExAmp cluster amplification workflow
  • Expert Video Tips on run setup
  • Instrument maintenance
  • Run quality interpretation.
  To access additional online training courses, go to the Training website, and under “Filters” select Training Type > Training and Videos > Online Training.
• Safety Data Sheets
  Safety Data Sheets contain information to help with appropriate reagent disposal practices. To obtain this information, enter the kit catalog number, language, and region/country, then select Search.

Additional technical resources are available 24/7 at HiSeq 3000 Sequencing System Support and HiSeq 4000 Sequencing System Support websites.