09/22/21
Illumina offers a wide variety of library preparation kits and sequencing platforms for different applications, each with its own coverage and indexing considerations. The table below lists the sequencing primers included in the NextSeq 1000/2000 cartridges, and the Illumina library preparation kits compatible with the NextSeq 1000/2000 platform. This information is based on the required data output and the indexing capabilities of each kit.
Note: The information in the table below is based on the required data output and indexing capabilities of each kit. Most Illumina libraries can be sequenced with all Illumina sequencing platforms. If a specific Illumina library is not listed below, contact Illumina Tech Support to discuss special considerations.
Refer to the Sequence Coverage Calculator and Library Prep and Array Kit Selector resources for additional information.
Table 1. Sequencing primer compatibility with Illumina library prep kits recommended for the NextSeq 1000/2000 sequencing platform.
| NextSeq 1000/2000 | |||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Read 1 Primer |
Index 1 (i7) Primer |
Index 2 (i5) Primer |
Read 2 Primer |
Library Type | |||||||||||||
| HP21 | BP14 | HP21 | AmpliSeq Panels Nextera DNA Nextera DNA Exome Illumina DNA Prep (M) Tagmentation (formerly Nextera DNA Flex Library Prep Kit) Illumina DNA Prep with Enrichment (S) Tagmentation (formerly Nextera Flex for Enrichment) Illumina Stranded mRNA, Ligation Illumina Stranded Total RNA with Ribo-Zero Plus, Ligation Illumina RNA Prep with Enrichment (L) Tagmentation Nextera Rapid Capture Custom Enrichment Nextera XT DNA TruSeq ChIP-Seq TruSeq DNA Methylation TruSeq DNA PCR-Free TruSeq DNA Exome TruSeq Methyl Capture EPIC TruSeq Nano DNA TruSeq RNA Exome TruSeq RNA v2 TruSeq Small RNA TruSeq Stranded Total RNA TruSeq Stranded mRNA TruSight Oncology UMI TruSight Cancer TruSight Cardio TruSight Inherited Disease TruSight Myeloid TruSight One TruSight RNA Fusion TruSight RNA Pan-Cancer TruSight Rapid Capture |
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For instructions on how to use custom primers on the NextSeq 1000/2000 platform, refer to the NextSeq 1000/2000 Custom Primer Guide.