09/21/21
Illumina Stranded RNA and TruSeq Stranded RNA library prep kits provide sequencing solutions for discovering, profiling, and quantifying RNA transcripts.
Stranded mRNA kits
The Illumina Stranded mRNA and TruSeq Stranded mRNA workflows capture mRNAs by poly-A selection and enable analysis of the coding transcriptome.
| Illumina Stranded mRNA Prep | TruSeq Stranded mRNA | |
| Input | Optimized for 25–1000 ng of high-quality total RNA | Optimized for 100–1000 ng of high-quality total RNA or 10–100 ng of previously isolated mRNA |
| Indexing | Uses ligation of index anchors to cDNA fragments and subsequent indexing PCR with unique dual indexes that enable multiplexing up to 384 libraries | Uses direct ligation of indexes to cDNA fragments with single indexes (24 indexes), combinatorial dual indexes (96 indexes), and unique dual indexes (96 indexes) |
Stranded Total RNA kits
The Illumina Stranded Total RNA and TruSeq Stranded Total RNA workflows retain RNAs following depletion of rRNA and abundant transcripts. These workflows enable analysis of the coding and noncoding transcriptome.
| Illumina Stranded Total RNA | TruSeq Stranded Total RNA | |
| Input | Optimized for 10–100 ng of RNA input; accepts 1–1000 ng purified total RNA input from high-quality RNA samples and 10–100 ng of RNA input from low-quality RNA samples or FFPE samples | Optimized for 100–1000 ng of total RNA and tested with 100 ng of RNA from FFPE samples |
| Indexing | Uses ligation of index anchors to cDNA fragments and subsequent indexing PCR with unique dual indexes that enable multiplexing up to 384 libraries | Uses direct ligation of indexes to cDNA fragments with single indexes (24 indexes), combinatorial dual indexes (96 indexes), and unique dual indexes (96 indexes) |
| Depletion of abundant transcripts | Uses Ribo-Zero Plus, an enzymatic depletion method that targets human, mouse, and rat cytoplasmic and mitochondrial rRNA, globin transcripts, and bacterial rRNA with one reagent | Uses Ribo-Zero, a pulldown-based depletion method that targets abundant transcripts:
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For more information on Illumina Ribo-Zero Plus depletion, refer to the rRNA Depletion using Illumina Ribo-Zero Plus bulletin.