Product Compatibility

Requirements & Compatibility

Sample Information

Use the following options to record information about your samples, such as sample ID and which index adapters are used for pooling. 

Library Preparation

See NextSeq Applications for possible applications.

After preparing libraries, denature and dilute libraries to a loading concentration of approximately 1.8 pM. For instructions, see NextSeq System Denature and Dilute Libraries Guide.

Kit Version and Control Software Compatibility

Kit Version

NCS Version

NextSeq 500/550 v2.5 Kit

NCS v2.2.0

NextSeq 500/550 v2 Kit

NCS v1.4, or later

NextSeq 500/550 Kit (v1)

NCS v1.3, or earlier

TG NextSeq 500 High Output Kit

NCS v1.3

Analysis Options

Additional Resources

Custom Protocol Selector—Generate customized end-to-end workflow instructions based on the Illumina products you use for library prep, sequencing, and analysis.

Library Prep Kit Selector—Helps you determine the best kit for your needs based on your project type, starting material, and the method or application. Includes recommended run parameters and analysis options.

Sequencing Coverage Calculator—Helps you determine the appropriate run parameters for the desired coverage for your experiment.