Nextera DNA Flex Library Prep Kit

Catalog IDs:  20018704, 20018705

This is the support page for Nextera DNA Flex for whole genome sequencing.  If running the Nextera Flex for Enrichment workflow including library prep and enrichment for whole exome sequencing, targeted enrichment, and targeted resequencing, refer to the support page for Nextera Flex for Enrichment.

Product Description

A fast, integrated workflow for a wide range of applications, from human whole-genome sequencing to amplicons, plasmids, and microbial species. This kit is compatible with Nextera DNA CD Indexes and IDT for Illumina Nextera DNA UD Indexes

Input Requirements

This protocol is optimized for 100–500 ng (large complex genomes) and as low as 1 ng (small genomes). For small genomes (<100 ng), use a fluorometric-based method to quantify input. For PCR cycling modifications according to input amount, see the Nextera DNA Flex Library Prep Reference Guide.