By the end of this course, you will be able to identify the Nextera DNA Flex kit, describe how the protocol works, identify what is needed to complete the protocol, and define the various steps in the library preparation workflow.
Many next-generation sequencing applications use the Polymerase Chain Reaction, or PCR, for DNA amplification. This video presents best practices to minimize the potential for PCR contamination in your experiment.
What are the impacts of sequencing amplicon and low diversity libraries on Illumina instruments? An Illumina Field Applications Scientist discusses considerations for designing and performing amplicon sequencing.
An Illumina Field Applications Scientist discusses best practices for sequencing amplicons on Illumina instruments with high-quality results. We use Sequencing Analysis Viewer (SAV) to compare key metrics of amplicon sequencing runs to a standard PhiX run.
Recorded Webinar (April 2019) | Illumina Technical Support invites you to join us for an introductory presentation on the Nextera DNA Flex library preparation kit. This webinar is geared towards new and intermediate users with a basic knowledge of next generation sequencing. This session covers sample to library workflow, and tips for a successful library prep.
Recorded Webinar (March 2019) | Join us to learn more about our newest and most efficient enrichment workflow– Nextera DNA Flex for Enrichment. This webinar is for users that are interested in targeted sequencing using enrichment with fixed and custom gene panels, as well as for current users of Illumina enrichment workflows to learn and compare the benefits Nextera DNA Flex for Enrichment offers. We will discuss the following topics: The improved enrichment workflow used in Nextera DNA Flex for Enrichment, the unique features of Nextera DNA Flex for Enrichment, and useful tips for a successful library prep.
CORRECTION: There is an update for low minor allele frequency (somatic variant) detection. Illumina has validated only 1-plex pooling for somatic variant detection. If you would like to pool above 1-plex for this application, you will need to validate the performance of higher plexity pooling.