Product Compatibility

Indexes

This kit is compatible with Nextera DNA CD Indexes and IDT for Illumina Nextera DNA UD Indexes

Sequencing

Recommended read lengths for sequencing on Illumina systems with Nextera CD Indexes (8 bp).

Sequencing System Read Length
HiSeq 2500, HiSeq 2000 (high output) 2 x 126
HiSeq 2500 (rapid run) 2 x 101
All others 2 x 151

IDT for Illumina Nextera DNA UD Indexes uses 10 base pair index codes, whereas Nextera DNA CD Indexes uses 8 base pair index codes. Therefore, you may need to adjust your sequencing setup. For more information, see IDT for Illumina Nextera DNA UD Indexes.