Nextera DNA Flex Compatible Products

Sample Information

Before preparing libraries, record information about your samples using an option available for your sequencing system.

Index Kits

This kit is compatible with Nextera DNA CD Indexes and IDT for Illumina Nextera DNA UD Indexes

Sequencing Guidelines

Nextera DNA CD Indexes

The following table provides the recommended read lengths for sequencing on Illumina systems with Nextera CD Indexes (8 bp).

Sequencing System Read Length
HiSeq 2500, HiSeq 2000 (high output) 2 x 126
HiSeq 2500 (rapid run) 2 x 101
All others 2 x 151
IDT for Illumina Nextera DNA UD Indexes

The following table provides the recommended number of cycles for sequencing on Illumina systems with IDT for Illumina Nextera DNA UD Indexes (10 bp).

For the MiniSeq and NextSeq Systems, the reagent cartride RFIDs encode a maximum number of cycles. The maximum value does not allow the full read length for Read 1 and Read 2 when each Index Read requires 10 cycles. Therefore, sequencing the full read length requires subtracting two cycles each from Read 1 and Read 2. Illumina expects to modify the RFID encoding to allow the extra sequencing cycles in the first half of 2019.

Sequencing System Read 1 Index 1 Index 2 Read 2

iSeq 100, MiSeq (v2/v3), HiSeq 2500/3000/4000, NovaSeq 6000

151

10

10

151

MiniSeq, NextSeq 500/550

149

10

10

149